Practical aspects of LC-ESI-MS/MS method development for analysis of biological matrices
Contenu
- Titre
- Practical aspects of LC-ESI-MS/MS method development for analysis of biological matrices
- Auteur
- Ledbetter, Thomas
- Résumé (fr)
-
L'objectif de ce stage était d'acquérir des compétences pratiques en analyse chimique grâce à l'observation et à la participation à deux projets de recherche distincts.
Dans le cadre de ce rapport, des exemples concrets tirés de chaque projet ont été utilisés pour aborder les instruments, la théorie et les techniques impliqués dans chaque étape de la chaîne analytique. - Abstract
-
The goal of this internship was to learn practical skills in chemical analysis through observation and involvement in two separate research projects.
For the purpose of this report, concrete examples from each project have been used
to discuss the instruments, theory and techniques implicated in each step of the
analytical chain.
For the “BNF project,” samples were extracted and derivatized before methods
were developed for flavonoid analytes using LC separation and ESI-MS/MS
detection with standards. Technical difficulties during the internship period
halted sample analysis so results from the “5-HIAA project” were used to discuss gradient development, calibration, and quantification methods.
Combined experience from both projects demonstrated that careful method development can reduce the need for extraction and clean-up of complex matrices while increasing the accuracy and precision of results. - Mot(s)-Clé(s)
- Analyse chimique
- High pressure liquid chromatography (HPLC)
- Fixation biologique de l'azote
- Croissance des plantes
- Symbiose plantes-bactéries
- Bactéries du sol
- Protéobactéries Rhizobia
- Légumineuses
- Céréales
- Chromatographie HPLC
- Tutelles
- UPPA-STEE
- Année de soutenance
- 2019
- Responsable(s) pédagogique(s)
- Ilag, Leopold
- Université de Stockholm
- Department of Environmental Science and Analytical Chemistry - Stockolm University
- Format
- application/pdf
- Langue
- eng
- Type
- Licence
- Diplôme + année
- Licence 3
- Mention
- Physique - Chimie
- Discipline principale
- Physique - Chimie
- Collections
- Physique - Chimie (L3PC)
Position : 9305 (10 vues)